Biotechnology 2 Labs

Biotechnology 2 Overview/Labs 

You will use two different online textbooks  for this class:


Text #1

Online Genetics Textbook: Download in a format you can read.

Text #2

Lab Notebook format:



Lab #1 Cell Membrane/Osmosis/ Diffusion Lab 


Standard Big Ideas in Biotechnology (Grade Level Expectations)   

1. Research and applications in the field of technology involve safe laboratory practices, science process skills, and use of appropriate technology.

2. Cellular metabolic activities are carried out by biomolecules produced by organisms.

3. Physical and behavioral characteristics of an organism are influenced to varying degrees by heritable genes, many of which encode instructions for the production of proteins.

4. Biotechnology can be applied as a possible solution to many problems facing human society, but the use of biotechnology can also have associated risks.  


Course Description

Students will explore the fundamental principles of biotechnology and business applications. Units of study include plant tissue culturing; plant and animal agriculture; DNA, RNA, and protein technologies; genetic diagnostics; healthcare and pharmaceuticals; food processing (GMO’s); fermentation technology; energy and environmental management; forensic science; cloning; stem cells; and bioethics. Laboratory activities reinforce concepts and principles presented.

Topics at a Glance

• Scientific inquiry

• Laboratory techniques

• Basic Biochemistry

• DNA structure and function

• Protein structure and function

• Biotechnology applications

• Bioethics

Content Area: Science - High School Biotechnology Prepared Graduates: Engage in scientific inquiry by asking or responding to scientifically oriented questions, collecting and analyzing data, giving priority to evidence, formulating explanations based on evidence, connecting explanations to scientific knowledge, and communicating and justifying explanations

GRADE LEVEL EXPECTATION Concepts and skills students master:  

1. Research and application in the field of technology involve safe laboratory practices, science process skills, and use of appropriate technology Evidence Outcomes 21st Century Skills and Readiness Competencies Students can:

a. Start and maintain a legal scientific notebook

b. Learn emergency procedures and the location of safety hazards and emergency equipment

c. Conduct a controlled experiment

d. Analyze and report data

e. Conduct a variety of basic procedures, tests and assays common to many biotechnology applications (use of a microscope, preparation of solutions, serial dilution, preparation of growth media, sterile technique, chromatography, gel electrophoresis, ELISA, spectrometry, etc.)

f. Understand and apply appropriate safety procedures

g.  Grow, maintain, and monitor bacteria and fungi 

h.  Learn microscope use for prepared and wet mount slides 

 Inquiry Questions: 

1. What types of questions and hypotheses can be answered by science?

2. What elements of design are critical in conducting a scientific investigation?

3. How can we ensure that scientific investigations are both safe and consistent with standard scientific practice?

4. What techniques and procedures are commonly used in biotechnology? (e.g., Cloning, DNA Sequencing, PCR, Electrophoresis, DNA fingerprinting, microarrays, ELISA, etc.)


Relevance and Application:

1. Biotechnology employs a wide variety of techniques that are applied to both basic research and applications.

2. Safe practices in the lab extend to safe practices in the workplace.

Nature of the Discipline:

1. The scientific method involves formulating a hypothesis, designing experiments to test the hypothesis, and evaluating the data to determine if the results support the hypothesis.

Content Area: Science - High School Biotechnology Standard: 

Analyze the relationship between structure and function in living systems at a variety of organizational levels, and recognize living systems’ dependence on natural selection

GRADE LEVEL EXPECTATION Concepts and skills students master:

Related questions and content:

Cellular metabolic activities are carried out by biomolecules produced by organisms

Evidence Outcomes 21st Century Skills and Readiness Competencies Students can:

a. Identify biomolecules such as carbohydrates, proteins and nucleic acids and their precursors/building blocks ►►►

b. Understand the structure and function of proteins and the role of enzymes as catalysts

c. Test enzyme activity at different concentrations or use a test to identify various macromolecules in solution

d. Describe the basic structure and function of DNA

e. Synthesize a compound using combinatorial chemistry Inquiry Questions:

1. How are rates of enzyme activity in cells affected by various factors such as concentration, pH or temperature? 

2. How does one know that enzymes speed up chemical reactions?

3. How does the shape of a protein relate to its function?

4. How does the structure of DNA support its functions?

5. How do cell signaling and cell division relate to the health of a cell/organism?

Relevance and Application:

1. Apply knowledge of biomolecular structure and activity to make consumer decisions, especially about diet with respect to saturated and unsaturated fatty acids, essential and nonessential amino acids, and simple and complex carbohydrates.

2. Recognize that many biomolecules can be made in the lab and have the exact same structure and function as ones made by living organisms.

3. Apply knowledge of cell signaling, cell division, and biomolecules to discuss possible treatments of cancer.

Nature of the Discipline:

1. Recognize that there are many career opportunities in the field of biotechnology. 

Materials: Bring all materials to class daily.

Three – Ring Binder (1.5” minimum) with 2 section dividers -- 1 for notes & 1 for quizzes
Journal notebook (small composition book)
Graph paper
Note cards
Pencils & Pens (Blue or Black ink only)
Calculator - preferably with scientific functions (scientific notation, logarithms, etc)
If any other items should be needed for labs, projects, etc.I will tell you in advance.

Grades: I will assess your understanding of chemical principles, problem-solving abilities, and other important skills through a variety of assignments.

Grading Scale:
            This course follows the Broward County Public Schools grading policy

            The distribution of the grades is:


                       Tests/Major Projects:40%

                       Homework, Classwork, Quizzes:  40%

                       Labs: 20%


Homework Checks: Sometimes I will check that you have completed your homework, without collecting it. 
Quizzes: You will have a number of quizzes throughout the semester (both for class content and for labs).  Sometimes they will be unannounced, so be prepared!!
Tests: Several will be given.
Lab Reports: You must complete the assigned labs by the assigned date.  For labs that do not require formal lab reports, I will ask you to hand in your data and results.  We may discuss these labs as a group.
Warm - Ups: Each day, you will be given an assignment to begin as you enter the classroom.  You should not wait for the bell to sound to begin this work. At any time, I may ask you to hand in the assignments from this section (be prepared to have them) for a grade. 

Missed Work: All assignments are due at the beginning of class unless told otherwise.

Absenteeism. Missing class is not an excuse for missing class material.   You must be responsible for finding out what you missed and you must catch up on all assignments and any quizzes or tests missed when you return to school.  

I do not provide the following items and you may wish to bring to class:

Hand Sanitizer
Clorox Wipes
Construction Paper
Graph Paper