AP Lab 5

Completing the Research Notebook for AP Biology Lab #5.....Cell Respiration

Resource: Lab Five, Cell Respiration

Page 54 in the AP Biology Lab Manual


Pre-lab: Complete the following parts in your research notebook prior to conducting the laboratory.


Part 1: Title

Develop a title for this lab in the form of a question after you have completed the pre-lab.


Part 2: Objectives (What are the objectives for this laboratory?)


Part 3: Pre-lab Questions

a. Define aerobic cellular respiration.

b. Write the complete chemical equation for cellular respiration.

c. Explain how cellular respiration will be measured in this lab.

d. p. 779 in text. Explain “dormancy” of a seed.

e. p 780 in text. Explain “imbibition” and “germination” in seeds.

f. What four concepts are apparent from the general gas law PV=nRT?

g. Seeds will produce carbon dioxide if they are respiring. How will this gas be removed from

enclosed respirometers?

h. After reading the method, describe how the three respirometers will be made.

i. How will the respirometer work? (page 55)

j. What is the purpose of the respirometer with glass beads?

k. Sketch the three respirometers and what will go in them.


Part 4: Method.

Three respirometers will be constructed. One will contain dry peas, one will contain germinating peas,

and one will contain glass beads. KOH will be used to chemically react with any carbon dioxide that

is produced from respiration in the respirometers. The respirometers will be submerges in room

temperature and 10 degree temperature. As oxygen is consumed in the respirometers, the gas

pressure decreases inside the respirometer, and water will enter the pipette. mL readings on the

pipette will give quantitative data which can be used to graph and calculate respiration rate in the

different environments.


a. If two respirometers are constructed that contain both nongerminating and germinating seeds at

the same temperature, then ______________________________________________________

b. If germinating seeds are placed in respirometers at both 10 degree temperature and room

temperature, then _____________________________________________________________


Part 5: Data: The data table is on page 58 of the lab handout.

Postlab work:

Page 59


6. Questions

Pages 59-61 Questions 1-14


Question 3 involves constructing a graph. Save this for the graph section.

Question 14 involves designing an experiment. Remember all the parts of a controlled experiment.


Part 7: Graph

Instructions are on page 60, Question # 3


Part 8: Theme Correlation

Energy Transfer

The topic of cellular respiration involves the metabolism of glucose, including

the reduction of molecular oxygen to produce water. Can you explain

examples of energy transfer throughout this whole process???

(Energy in glucose is ultimately transferred to what?)


How was this study controlled?


Part 9: Conclusion.

What is cellular respiration (not just a definition, but chemically, what occurs?)

Why could this experimental set up work?

What factors affect the rate of respiration according to this study?

Use specific data and analysis to discuss rate of respiration.